In this part of series of articles I will try to explain you how you can Perform Subtration operation on two images using C#. Create a new application using C#.net. and drag two picture box and open file dialog on the from. Create a "File" menu with "New" as a sub menu having Image 1,Image 2 as a sub menu, "Image" Menu with "Stretch" and "Center" as a sub menu and "Two Source Filters" with the "Subtract" as sub menu. As we have to us the pointers in it so we have to set the configuration properties of the project.